Human Platelet - Derived Mitogens . I . Identification of Insulinlike Growth
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چکیده
Human platelet lysates contained potent mitogenic activities for MCF-7 human breast-cancer cells in serum-freedefined media. Because these activities were not replaced by known platelet mitogens. such as platelet-derived growth factor or transforming growth factor , we sought to identify the breast cancer cell mitogens by purification and N amino-acid sequencing. Acetic acid extracts of outdated human platelets were concentrated by ammonium sulfate precipitation and fractionated on Sephadex G-50 and Bio-Gel P-b columns in 0.5 mol/L acetic acid. Two major activities were resolved by molecular sieve methods and fractionated further by reverse-phase highperformance liquid chromatography (HPLC). Purifications (70.000 to 870.000-fold) were accomplished yielding mol
منابع مشابه
Human platelet-derived mitogens. I. Identification of insulinlike growth factors I and II by purification and N alpha amino acid sequence analysis.
Human platelet lysates contained potent mitogenic activities for MCF-7 human breast-cancer cells in serum-free-defined media. Because these activities were not replaced by known platelet mitogens, such as platelet-derived growth factor or transforming growth factor beta, we sought to identify the breast cancer cell mitogens by purification and N alpha amino-acid sequencing. Acetic acid extracts...
متن کاملHuman Platelet - Derived Mitogens . I . Identification of Insulinlike Growth Factors I and II by Purification and N # { 176 } Amino Acid Sequence Analysis
Human platelet lysates contained potent mitogenic activities for MCF-7 human breast-cancer cells in serum-freedefined media. Because these activities were not replaced by known platelet mitogens. such as platelet-derived growth factor or transforming growth factor , we sought to identify the breast cancer cell mitogens by purification and N amino-acid sequencing. Acetic acid extracts of outdate...
متن کاملHuman Platelet - Derived Mitogens . II . Subcellular Localization of Insulinlike Growth Factor I to the a - Granule and Release in Response to Thrombin By Kenneth
Platelets contain mitogenic activities for MCF-7 human breast cancer cells when assayed under serum-free chemically defined conditions. Purification from outdated human platelets identified insulinlike growth factor I (IGF-I) as the most potent breast cancer cell mitogen in lysates (Karey KP, Sirbasku DA: see accompanying article, this issue). In this study the release and subcellular localizat...
متن کاملHuman Platelet - Derived Mitogens . II . Subcellular Localization of Insulinlike Growth Factor I to the a - Granule and Release in Response to Thrombin
Platelets contain mitogenic activities for MCF-7 human breast cancer cells when assayed under serum-free chemically defined conditions. Purification from outdated human platelets identified insulinlike growth factor I (IGF-I) as the most potent breast cancer cell mitogen in lysates (Karey KP, Sirbasku DA: see accompanying article, this issue). In this study the release and subcellular localizat...
متن کاملHuman platelet-derived mitogens. II. Subcellular localization of insulinlike growth factor I to the alpha-granule and release in response to thrombin.
Platelets contain mitogenic activities for MCF-7 human breast cancer cells when assayed under serum-free chemically defined conditions. Purification from outdated human platelets identified insulinlike growth factor I (IGF-I) as the most potent breast cancer cell mitogen in lysates (Karey KP, Sirbasku DA: see accompanying article, this issue). In this study the release and subcellular localizat...
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